Primer

Part:BBa_K4344039

Designed by: Marcel Pott   Group: iGEM22_Heidelberg   (2022-09-29)


p19-Tac Extension-fwd.

forward primer for tac-polymerase extension

Usage and Biology

Selection of results obtained during the engineering process of pUC19-p19-siRNA UL19..E: Analytical gel of pUC19-p19-siRNA UL19 after insertion tac-LacO-Shine Dalgarno extension. F: Proposed structure of the complete pUC19-p19-siRNA UL19 (tac-LacO-SD).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None